Translational_Unit

Part:BBa_K322123

Designed by: Chris French and Richard Edward Partridge-Hicks   Group: iGEM10_Edinburgh   (2010-09-25)

Phycobilin synthesis operon without terminator

Phycobilin is chromophores (light-capturing molecules) found in cyanobacteria and in the chloroplasts of red algae, glaucophytes and some cryptomonads (though not in green algae and higher plants)， used in light sensor. It is derived from K098010 but PCR was used to remove the terminator, so that cph8 could be added at the end of the operon. The there is a weak RBS (B0032) before the heme oxygenase gene and a strong RBS (B0034) before the pcyA gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 644
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 971
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None